The best time of year to apply beneficial nematodes is late spring or early fall. These two windows line up with peak pest activity in the soil and give nematodes the warm ground they need to hunt. Your exact timing depends on your climate zone and the pests you're after.
I found out the hard way that rushing a spring round doesn't pay off. One March I got excited and sprayed nematodes onto beds that were still at 45°F (7°C). Three weeks later the grub damage looked the same. The next year I waited until mid-May when the soil hit 65°F (18°C) and the results were night and day. Grub damage stopped in two weeks and my lawn bounced back fast.
So when to apply nematodes comes down to soil heat. Most species work best at 68-86°F (20-30°C) in the ground. Below 50°F (10°C) they get sluggish and stop hunting. Between those two ranges they still work but at a slower pace. Early fall in most areas gives you a solid window while the soil holds summer warmth.
Your nematode application season also depends on where you live. Southern growers in zones 8-10 can treat from March through November. Northern growers in zones 3-5 have a tighter window from May through September. Coastal spots hold mild soil temps longer into fall. Always check your own soil rather than going by dates on a calendar.
Spring rounds target pest larvae that spent the winter in your soil. These bugs come up near the surface as the ground warms and that's when nematodes can reach them. Fall rounds catch grubs and crane fly larvae while they still feed in the root zone. By late fall these pests dig too deep for nematodes to find them. Hitting them before they go deep gives you much better kill rates.
In my experience, two rounds per year give you the best results. I do one in late May and one in early September. This covers both waves of pest activity and keeps my lawn grub-free all season. Grab a soil probe and push it 2-4 inches into the ground to check your temp. Once you read 55°F (13°C) or above, most species will work well for you.
For cold weather use, pick S. kraussei. This species stays active down to 41°F (5°C) when every other type has shut down. It's your only option for late fall and early spring in cold zones. Match your species to your soil temp and your target pest. Every dollar you spend on nematodes pays you back with fewer pests and healthier turf.
Read the full article: Beneficial Nematodes Pest Control Guide