Seed Viability Test Guide: Ensure Your Seeds Grow

Written by
Paul Reynolds
Reviewed by
Prof. Samuel Fitzgerald, Ph.D.A seed viability test assesses whether your seeds will germinate pre-planting.
Water testing reveals non-viable seeds using observance of simple buoyancy.
Tetrazolium staining reveals living tissue by using color reactions in the chemical.
Germination tests predict rates of germination in controlled environments with towels.
Viability percentages allow seed density-planning.
Regular testing can save you wasting your effort on seeds that may or may not sprout.
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A seed viability test is used to determine if your seeds can sprout and grow into plants. This biological potential depends on the conditions under which the seeds are stored as well as the age of the seed. Suppose you test your seeds before planting them. In that case, there is no wasted effort on seeds that lack the capability to germinate. You save the work of planting and the time spent softening the soil for planting by determining which seeds are worth growing.
This guide includes both home techniques that are easy to do and more scientific lab methods. Both methods will help to determine which seeds are alive. I test my seeds every year. Twelve years ago, I lost my entire crop of tomatoes because of old seed. If you examine your seeds, you can be assured that your garden will be productive and that the yield will be good.
Utilize these dependable techniques to avoid planting duds. Testing gives each seed planted the greatest opportunity to thrive. Your success as a gardener begins with understanding seed viability. Knowing this alters how you regard each planting season.
Water Test Method
The water test method is the simplest way to test seed viability with just water and a container. This method is suitable for large seeds, such as beans, peas, and corn, and quickly indicates a lack of density among the seeds. Fill a bowl with water and drop your seeds into it, noting which ones sink and which ones float after 15 minutes.
The reason most good seeds sink is that they have solid and dense interiors, while the hollow and decayed seeds float. Exceptions to the floating rule are possible. Viable corn seeds often float, due to air caverns in their structure. Once, I threw out a lot of floating corn seeds, thinking that they were worthless; I later learned that they were good seeds and could have sprouted. A germination test should always verify the results of the water test to conform to actual conditions.
This method is not as effective for small seeds, such as carrots and lettuce, as the buoyancy of the seeds does not provide a reliable test. Their lightness causes them to float regardless of whether they are viable or not. With these, use the germination test. Testing seeds in water is a useful preliminary test to determine which seeds will be most satisfactory before committing space to them in the garden.
This rapid assessment helps avoid planting duds, although it has its limitations. Use in conjunction with other methods when viability is uncertain. Good testing will prevent wasted rows in your garden. It gives you the confidence to know which varieties are worth planting with your effort and resources.
Tetrazolium Test Method
The tetrazolium test method uses biochemistry to determine seed viability. Living cells convert colorless tetrazolium chloride into color-forming red formazan dye. This biochemical reaction only occurs in metabolically active tissues. Examination of sown seeds reveals that viable embryos exhibit bright pink or red colors. This technique gives more scientific accuracy than simple germination tests.
Seeds should be cut in half lengthwise for a good examination of the staining. In this way, the internal tissues are brought into contact with each embryo. For further searching of the internal parts, I always employ a sharp knife and a lens. Great care should be taken when handling the tetrazolium solution, and gloves should always be worn during the operation in well-ventilated rooms.
Safety is the number one priority when handling chemicals. Use chemical-resistant gloves and protective eye wear. The unused tetrazolium solution should be stored in dark glass containers that prevent light from reaching it. Refrigeration considerably extends the useful life of the solution. Proper storage prevents deterioration of this sensitive testing solution.
This test reveals cell activity not apparent by other means. The presence of bright red staining proves the presence of living tissue, while the pale areas show dead cells. All the cells that were stained partly indicate reduced viability. You get actual biological proof of the potential of each seed before planting.
Preparation
- Solution: Prepare 1% tetrazolium chloride solution using distilled water
- Safety: Wear gloves and work in ventilated area to avoid skin contact
- Materials: Scalpel, Petri dishes, white paper for observation
Seed Preparation
- Soaking: Submerge seeds in water for 12-24 hours to soften tissues
- Dissection: Carefully cut through seed midpoint using scalpel
- Handling: Avoid damaging embryo during cutting process
Staining Process
- Immersion: Completely cover seed halves with TZ solution
- Duration: Maintain at 86°F (30°C) for 2-4 hours in darkness
- Observation: Check every 30 minutes for color development
Result Interpretation
- Positive: Living tissues stain bright red or pink
- Negative: Dead tissues remain colorless or pale
- Partial: Patchy staining indicates reduced viability
Cleanup
- Disposal: Neutralize solution with baking soda before draining
- Storage: Keep unused TZ solution in amber bottle at 40°F (4°C)
- Tools: Rinse dissection tools immediately after use
Expected Viability by Seed Type
Different seeds have different natural life cycles under ideal storage conditions. A one- or two-year life for onion and parsley seed, but for lettuce and tomato seed, a five-year life. Such periods require a cool, dry, low-humidity environment, with temperatures not warmer than 40°F. (4°C). Seeds should always be stored in air-tight containers.
Test seed germination when nearing their viability limits. Corn at 2 years of age may germinate, but at a slower rate. I test my carrot seeds each year, as I've experienced crop loss due to expired seeds. Reliable testing alleviates plant disappointment in mid-season. Be aware of the expiration dates of your seeds.
Common herbs such as basil and cilantro have a life span of 3-5 years with proper storage. Vegetables such as beans and peppers typically last 2 to 3 years. It is a good idea to group your seeds by category so that you can easily reference them; this way, you can plan out when to conduct your tests.
Don't dismiss older seeds. If tomatoes have been stored correctly, expect 50% germination after five years or more. Adjust density according to the tests. The success of your garden depends upon this knowledge of viability. Plan your planting around known seed vitality.
Germination Test Method
The germination test method uses common household goods: paper towels, resealable bags, and water. With these inexpensive tools, virtually anyone can test seeds before planting them. Moisten a paper towel, lay the seeds upon them, and place the towels in a resalable plastic bag that has a proper label. The equipment involved costs almost nothing, yet the test gives reasonably reliable results.
Optimal temperatures of 70-80°F (21-27°C) are required for good germination. Most seeds germinate well in the dark, so a steady countertop works well. Tomatoes prefer constant warmth, while lettuce must have slightly cooler temperatures. I keep my bags on top of the refrigerator for continuous warmth.
To prevent mold, ensure the towels are damp but not soaking wet. Check for fungus infections daily and eliminate any infected seedlings immediately. Some plants, such as carrot seedlings, require a longer incubation period, up to 14 days. It pays to be patient while waiting for reasonable accuracy in decisions regarding viability.
Clearly label every bag with the seed variety and start date. One mix will spoil your data. I once confused pepper seeds with tomato, returning no results of value. Correct identification of the seeds provides accurate data for each variety.
This examination accurately demonstrates sprouting abilities as opposed to theoretical processes. You observe the root emergence and correctly quantify actual germination rates. You can modify planting density with real measurements. Garden success increases from this editorial verification.
Materials Needed
- Paper towels: Use plain white towels without prints or dyes
- Resealable bags: Clear plastic bags for visibility and moisture retention
- Labels: Waterproof marker for identifying seed varieties
- Spray bottle: For remoistening towels without disturbing seeds
Seed Preparation
- Quantity: 10-20 seeds per variety for statistical reliability
- Selection: Choose undamaged seeds representing the entire batch
- Grouping: Keep different varieties on separate towels
- Pre-treatment: No soaking required for most seeds
Environment Control
- Temperature: Maintain 70-80°F (21-27°C) for optimal results
- Light: Most seeds germinate in darkness; avoid direct sunlight
- Surface: Use stable, non-porous countertop or shelf
- Consistency: Avoid moving bags during testing period
Moisture Management
- Initial dampness: Towels should be moist but not dripping
- Maintenance: Check daily; remoisten if >50% dryness occurs
- Problem signs: Discard test if mold covers >25% of surface
- Alternative: Use distilled water to prevent mineral deposits
Observation Protocol
- Frequency: Check daily after day 2 for fast-germinating seeds
- Documentation: Record sprout counts and dates in a log
- Termination: End test when no new sprouts appear for 3 days
- Handling: Use tweezers to examine seeds without contamination
Calculating Germination Rate
Rate of germination is the percentage of seeds that successfully sprout. It can be calculated by dividing the number of seeds that grow by the total seeds tested and multiplying by 100. For instance, if out of ten seeds tested, eight sprout, then your germination rate is 80%. This figure will help you make informed decisions about the amount of seed to plant.
Try this computation using various quantities of seeds. Letting 20 seeds be tested, where 15 sprout, gives (15/20) x 100 = 75%. With 10 seeds and 7 sprouts, it provides 70%. I have the growth proportion for every lot of seeds each season. This habit is worth considering to prevent failures in planting.
Adjust your planting plans accordingly based on the results. A planting rate above 90% requires normal spacing. For planting rates between 50% and 89%, plant 1.5 times the number of seeds required for normal planting. If the planting rates are under 50% consider either replacing the seeds or heavy overplanting with seeds. This will increase the efficiency of your garden.
Record final counts only after a period of 48 hours without any new sprouts. Do not include any seeds that have started to mold in your totals. Accurate data is essential to ensure the reliability of our calculations. This step takes us from test results to developing an actionable plan for the garden to maximize yield.
Count Sprouted Seeds
- Definition: Only count seeds with visible root/shoot emergence
- Timing: Final count when no new sprouts appear for 48 hours
- Exclusions: Discard moldy or contaminated seeds from count
Record Total Seeds Tested
- Baseline: Use initial seed count before testing began
- Accuracy: Note if any seeds were removed (e.g., mold contamination)
- Standardization: Minimum 10 seeds recommended for reliability
Apply Calculation Formula
- Equation: (Number of sprouted seeds ÷ Total seeds) x 100
- Example 1: 8 sprouts from 10 seeds = (8÷10)x100 = 80%
- Example 2: 15 sprouts from 20 seeds = (15÷20)x100 = 75%
Interpret Percentage Results
- 90-100%: Excellent viability (plant at normal density)
- 50-89%: Good viability (sow 1.5x extra seeds)
- <50%: Poor viability (replace seeds or sow 2x density)
Adjust Planting Strategy
- High viability: Follow standard spacing guidelines
- Moderate viability: Increase seed quantity by 25-50%
- Low viability: Sow thickly or source new seeds
5 Common Myths
Discard all seeds that float in the water test.
All seeds that float in the water test, are unviable. Some seeds are to be found in good condition, and viable, which have escaped the test process by floating, due to air cells in their coats and structure. This is very likely to be the case with corn and sunflower seeds, to say nothing of other seeds whose coats have natural air cells in them. Make certain that the water test is confirmed by the germination test before discarding seeds. It is possible that such seeds as possibly have been planted, may be incorrectly judged as unviable by the water test alone.
Naturally, seeds lose their viability when kept frozen and should not be kept frozen.
@@Well-dried seeds may easily be treated to freezing temperatures and will actually thrive on the cold treatment when employed for long-termed preservation. The important thing is to have it perfectly dry. The seeds should be dried down to below 8% moisture before freezing, otherwise, the injurious effects of freezing may result through the formation of ice crystals which may break the cells of the seed. Many more or less professional seed men use a freezing temperature of 0° F. (-18C) as a matter of course and seeds like tomatoes and peppers will retain their vitality in 10 years if subjected to freezing heat.
Seeds that don't make it past their age of viability are completely worthless and they will not grow.
The age of viability represents average expectation under ideal conditions, but not death dates. Seeds do not become completely dead, but become gradually less vigorous and many will still sprout after the age of viability, though not at the same percentage. For instance, properly stored tomato seeds frequently show 30-50 per cent. germination after safe age of 7 years. Always determine the age of the seeds instead of discarding them, as they may yet be valuable, with different planting density giving good germination.
Complete darkness is absolutely essential for successful germination testing.
While darkness benefits some species, the critical factors for germination are consistent moisture and optimal temperature between 70-85F (21-29C). Many common garden seeds like lettuce and tomatoes actually germinate better with light exposure. The key is maintaining stable conditions rather than obsessing over light exclusion, as temperature fluctuations and moisture inconsistency cause more test failures than light exposure ever would.
Tetrazolium testing has to be done in a lab and not at home.
Professional laboratories use standardized formulations, but home gardeners can perform simplified tetrazolium tests with ease, as they can obtain these 1% solutions by going through agricultural suppliers. It requires careful dissection and observation of seeds rather than sophisticated equipment, and many of the gardening communities online have proven successful home testing techniques. When practiced with the proper precautions (gloves, ventilation), this scientific method is available for determining the viability of seeds to the home gardener without resorting to the services of a professional laboratory.
Conclusion
Checking seed viability saves unnecessary labor and expense in the garden. Simple tests, such as water tests and germination checks, prevent you from planting seeds or plants that will not sprout. I have seen many gardeners lose a whole season because they had not taken this precaution. Why not protect your time and investment with this reliable testing method?
Since different seeds and conditions require other tests, water tests are used for quick corn and bean tests, germination tests provide accurate counts, and tetrazolium tests are used for scientific purposes. If the test matches the seeds you want to test, it will yield the best results. Use the tests you prefer.
Viability testing enables you to make informed decisions about gardening. Knowledge of seed germination allows you to adjust planting density and avoid complete crop failures. This intelligence transforms uncertainty into clear planning for each seed planted in the garden, where success begins.
Let seed viability testing become a part of your seasonal gardening schedule. Before each planting season, do a little seed viability testing. Establish this habit. Maximize your garden's yield and avoid disappointment. Start testing your seeds.Transform your gardening approach until your plants reward you with bountiful harvests.
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Frequently Asked Questions
How do you test seed viability?
The three primary seed viability test methods are the water test (buoyancy check), germination test (controlled sprouting), and tetrazolium test (chemical staining). Each method identifies viable seeds through different principles: density observation, actual growth demonstration, or cellular activity detection in seed embryos.
Do viable seeds sink or float?
In water viability tests, most viable seeds sink while non-viable seeds float due to hollow cavities or low density. However, some dense viable seeds like corn may float because of trapped air pockets, so always confirm with a germination test for accuracy.
Can old seeds still germinate?
Old seeds often retain partial viability beyond their labeled expiration if stored properly in cool, dry conditions. Viability gradually declines rather than disappearing completely - for example, tomato seeds can show 30-50% germination after 7+ years. Always perform a viability test before planting older seeds.
What chemical tests seed viability?
Tetrazolium chloride solution is used to test seed viability through cellular staining. Living seed tissue turns red when exposed to this chemical, while dead tissue remains colorless. This method requires careful seed dissection and observation under controlled temperature conditions.
Does freezing damage seeds?
Properly dried seeds can be safely frozen for long-term storage without damage. The critical factor is moisture content - seeds must be below 8% moisture before freezing to prevent ice crystal formation. Many seed banks use freezing at 0°F (-18°C) for preservation.
How accurate are seed viability tests?
Viability tests provide reliable indicators when properly conducted: germination tests show actual growth potential, water tests offer quick density checks, and tetrazolium reveals cellular activity. For best accuracy, test multiple seeds per batch and combine methods when viability appears borderline.
What seeds are illegal to save?
Patented hybrid seeds and some genetically modified varieties often have legal restrictions on saving and replanting. These restrictions protect plant breeders' intellectual property. Always check seed packaging for specific terms before saving seeds from protected plant varieties.
Can seeds germinate after decades?
Certain seeds like lotus or date palms have germinated after centuries in controlled conditions, but most garden seeds lose viability within 1-10 years. Extreme longevity requires perfect storage conditions and specific seed biology - don't assume old seeds will sprout without testing.
What affects seed viability most?
Three primary factors impact seed viability: storage temperature (ideal 35-40°F), humidity levels (below 40% RH), and seed age. Oxygen exposure and light also contribute to deterioration. Properly stored seeds in airtight containers maintain viability longest.
How do you interpret germination rates?
Germination rates indicate planting strategy: 90-100% requires normal density, 50-89% needs 1.5x more seeds, and below 50% suggests replacement or heavy overplanting. Calculate by dividing sprouted seeds by total tested seeds, then multiplying by 100 for percentage.